TP53 and familial pancreatic carcinoma: To assess whether ROS-activated mutp53 contributes to the regulation of the miR-135 family, we knocked down mutp53 in MIA PaCa-2 cells and found that the upregulation of miR-135a and miR-135b upon glutamine deprivation was largely attenuated in p53 knockdown cells (Fig. 2f, g), suggesting mutp53 in pancreatic cancer cells displays a gain-of-function in response to the metabolic stress.