We find that H19 significantly induces let-7a-5p/7d-5p/7f-5p in cholestatic mouse livers and facilitates the interactions between PTBP1 and pre-let-7d/pre-let-7–7a-1 in vitro, suggesting that the PTBP1/let-7 miRNAs may be involved in the progression of cholestasis. This evidence concerns the gene PTBP1 and cholestasis.