Two HCC cell lines Huh7 and Hepa1, both of which contained detectable levels of let-7a-5p/7d-5p, were transfected with psiCHECK2-let-7 4× together with empty pcDNA3, PTBP1, or H19 plasmid. This evidence concerns the gene PTBP1 and hepatocellular carcinoma.