ACP5 and hydrops fetalis: Next, bone regeneration in the four groups was detected by HE staining, Masson staining, IHC of ALP, and TRAP staining, the results showed the following: (1) compared with NC group, bone regeneration was inhibited in HF, HG, and HFHF groups; (2) compared with other three groups, the inhibition of bone regeneration was the highest in HFHG group.