To analyze cytokine secretion by M28z10 T cells after target cell stimulation, we collected the culture supernatant of BGC-823-GL tumor cells in a killing assay and then detected cytokines and effector molecules that are generally secreted by activated T cells, including IL-2, interferon-γ (IFN-γ), granulocyte-macrophage colony-stimulating factor (GM-CSF), and granzyme B, by enzyme-linked immune absorbance assay (ELISA). Here, GZMB is linked to neoplasm.