To redirect human T cells to the MSLN antigen expressed by GC tumor cells, we constructed the third-generation M28z10 vector containing the scFv that recognizes MSLN, CD28 transmembrane domain, CD3ζ T cell activating domain, and the costimulatory domains from both CD28 and DAP10 as previously described [23, 36]. The gene discussed is HCST; the disease is neoplasm.