In the current study, we sought to examine 1) if these typically reproductive neuropeptides (kisspeptin and GnRH) are synthesized in canine osteosarcoma cell lines, 2) if GnRH may be secreted by these tumor cells, and 3) what effects autocrine production of these peptides could have on tumor growth and bone remodeling physiology. Here, KISS1 is linked to osteosarcoma.