To explore if canine osteosarcoma cells may employ reproductive neurohormones and their receptors as autocrine/paracrine factors, we probed established tumor cell lines for expression of canine gnrh and kiss1, and their respective cognate receptors gnrhr and gpr54/kiss1R, using RT-PCR (Fig. 1a-d). The gene discussed is KISS1; the disease is osteosarcoma.