In this study, we used the adjuvant Pam3CSK4 in the DPX-rPA formulation, as it also induces strong antibody responses,18 and was used clinically in another infectious disease-based project with DPX making it a more clinically attractive component.14 As a comparator, we utilized the commercially available AVA vaccine instead of alum-rPA. This evidence concerns the gene RPA1 and infectious disease.