To determine the long-term effect of TRAIL and HRK cooperation in LN18 and U87MG cells, we employed real-time cell growth assays and acutely transduced GBM cells with GFP or HRK encoding viruses (time designated as t1) followed by medium change (t2) and TRAIL treatment (t3). This evidence concerns the gene TNFSF10 and glioblastoma.