Barceló-Batllori et al. evaluated homogenates from DLD-1 colorectal adenocarcinoma cells (as in vitro IBD model) before and after exposure to IFN-γ, IL-6, and IL-1 and utilised 2D PAGE together with MALDI-TOF-MS in a search for cytokine-regulated proteins in intestinal epithelial cells (IEC) in IBD [131]. This evidence concerns the gene IL6 and inflammatory bowel disease.