To further evaluate the role of PCAT1 in castration-resistant growth of PCa, we generated and characterized an androgen-independent LNCaP-AI cell line by long-term culture of androgen-dependent LNCaP cells in RPMI-1640 medium containing charcoal-stripped serum (Figure 1F and Supplementary Figure S1A–C). The gene discussed is PCAT1; the disease is posterior cortical atrophy.