As the full-length sequence of EGFR-AS1 was obtained by 5′ and 3′ RACE experiments, EGFR-AS1 overexpression (lv-oeEGFR-AS1) and control lentivirus (lv-NC) were constructed and transfected into RCC cell lines (Supplementary Figure S2d). Here, EGFR is linked to renal cell carcinoma.