For example, although high intratumor PD-L1 expression seems to be significantly associated with a better response to PD-1/PD-L1 blockade agents in several tumors [27], the spatial heterogeneity and dynamic changes of expression in the same tumor, together with the lack of reliable detection methods and definite cut-off values, actually limits its widespread use in clinical practice. This evidence concerns the gene CD274 and neoplasm.