As previously reported [12,32], this assay assessed the gene expression of melanoma specific genes, MLANA/MART-1 (melanoma antigen recognized by T cells), TYR (tyrosinase), MAGEA3 (melanoma antigen family A3), PAX3 (paired box protein Pax-3 isoform 3), and ABCB5 at a sensitivity level of at least one melanoma cell in a background of 1 × 105 WBCs. The gene discussed is ABCB5; the disease is melanoma.