In accordance with both our hypothesis and findings, several studies have already demonstrated a tumour suppressor function of prdxs in HCC.24–26 Given the ability of carcinogenic cells to reprogramme their metabolism towards aerobic glycolysis and the lack of studies especially on Aco1 and Mdh1 in HCC, we chose three proteins of the TCA cycle for validation of 2D-DIGE-based proteomic results. The gene discussed is ACO1; the disease is hepatocellular carcinoma.