NOTCH3 and cholangiocarcinoma: Notch3 expression has been poorly investigated with different results in CCA.27,28 Herein we analysed Notch3 expression in 60 CCA and in matched non-tumour tissues; we found that it was aberrantly expressed in 75% of patients in malignant ductules, with a nuclear localisation suggesting its functional role (Fig. 6a).