Although the previous reports have shown that only Kunitz domain 1 of HAI-2 is responsible for inhibiting HGFA, matriptase and prostate cancer cell invasion,39,57 we find that both Kunitz domains of HAI-2 can suppress plasmin activities (Supplementary Figure S16A and S16B) and cell motility (Supplementary Figure S16C and S16D) in NSCLC. This evidence concerns the gene SPINT2 and Familial prostate cancer.