The usage of both the UL52–UL54 (Fig. 6a) and RL2–UL1 (Fig. 7a) splice junctions can be readily detected by end-point RT-PCR, albeit at lower levels than their non-fusion forms, using RNA collected from NHDFs infected by multiple HSV-1 strains at 18 h post infection (Figs. 6b and 7b). This evidence concerns the gene RPL10A and infection.