NPPB and early-onset autosomal dominant Alzheimer disease: In contrast, a study by Hu and colleagues examined BNP levels in the Alzheimer's Disease Neuroimaging Initiative (ADNI) cohort, which consisted of control or healthy subjects (N = 58), subjects diagnosed with MCI (N = 396) and subjects diagnosed with AD (N = 112), by using a multiplexed immunoassay (Rules-Based Medicine, Austin, TX, USA), and found elevated levels of BNP in patients with clinical MCI/AD compared to healthy, control subjects [28].