We found that the siRNA‐induced knockdown of SSRP1 significantly inhibited cell cycle progression from G0/G1 phase to S phase in CRC cells, resulting in G1 cell cycle arrest due to the reduction in cyclin D1 and 14‐3‐3 expression and the increase in p53, p21 and p27 expression. The gene discussed is SSRP1; the disease is colorectal carcinoma.