Besides, we conducted colony-forming unit assays, EdU experiments, western blotting, and immunofluorescence (IF) to evaluate the role of EYA2 in tumor proliferation and explore EYA2 regulated genes Finally, we employed the Kaplan-Meier Plotter platform to explore the role of EYA2 in the prognosis of breast cancer patients. The gene discussed is EYA2; the disease is breast carcinoma.