Herein, we determined whether activation of NK cells isolated from thawed peripheral blood mononuclear cell (PBMC) preparations derived from patients with prostate cancer by either co-incubation with mitomycin C treated CTV-1 cells or stimulation with IL-2 enhanced their capacity to kill the human metastatic disease-derived prostate cancer cell line PC3. Here, IL2 is linked to Familial prostate cancer.