Initial studies using endpoint PCR approaches detected the presence of this allele in the cfDNA of Ad5-CMV-Cre-infected Kras+/LSL-G12D mice at 12 weeks p.i. (R. M. Trigg, Molecular analysis of circulating cell-free DNA in lung cancer, PhD thesis, University of Leicester, 2017), encouraging the development of a qPCR assay. Here, KRAS is linked to lung cancer.