Conversely, MANF induced by the stimulation of cerebral ischemia had no overlap with DAPI, suggesting that MANF localizes in cytosol and focal ischemia did not induce its relocalization [29], whereas nuclear MANF immune-positive signals were observed in a few TM or LPS-treated primarily cultured fibroblast-like synoviocytes as well as in the synovial tissue of antigen-induced arthritis rabbit model [23]. The gene discussed is MANF; the disease is brain ischemia.