To investigate the effects of AKE and amygdalin on inflammatory factors, including tumor necrosis factor (TNF)-α and interleukin (IL)-6, enzyme-linked immunosorbent assay (ELISA) and quantitative polymerase chain reaction (qPCR) were performed in conjunctival epithelial cells. Here, IL6 is linked to punctate palmoplantar keratoderma type III.