The efficient and robust activation of immune response following infection with VACV-Wyeth, continued in later days and on four to five d.p.i., induction of related pathways as CD28 in T Helper Cells, Communication between innate and adaptive immune cells and T cell receptor signaling were observed (Supplementary Figure S1, VACV-Wyeth pathways # 15, 21, 26, respectively). Here, CD28 is linked to infection.