SIRT5 and persistent truncus arteriosus: Echocardiographic measurement was used to determine the structures and hemodynamics of left ventricle and aortic arch with heart rate being kept to around 500 bpm in all four groups (i.e. WT-Sham, WT-TAC, Sirt5 KO-Sham, Sirt5 KO-TAC) (Table 1).