Here we screened 481 pediatric BCP-ALL cases at initial diagnosis, 19 relapse BCP-ALL cases, and 12 BCP-ALL cell lines for variants in FGFR1, FGFR2, and FGFR3. We found a low frequency of variants among patients at initial diagnosis, and no variants in the relapse cohort or in cell lines. Here, FGFR1 is linked to acute lymphoblastic leukemia.