In an additional independent UBC cohort (n = 196, Supplementary Table 6), extracting paraffin-embedded tumor tissue DNA and Sanger sequencing the enhancer of ADGRG6 detected the similar mutational spectrum and higher mutational incidence, which might be due to the increased purity of tumor cells in tissue sections and the enhanced accuracy of Sanger sequencing (Fig. 3a). Here, ADGRG6 is linked to neoplasm.