To confirm DRAM2 was overexpressed in tumor tissue, we analyzed DRAM2 mRNA expression in 20 pairs of fresh NSCLC samples via RT-PCR (Fig. 1c) and 15 pairs of tissue specimens, which corresponded to RT-PCR to analyze DRAM2 expression through western blotting (Fig. 1d), and obtained the same results. This evidence concerns the gene DRAM2 and neoplasm.