ALK and neoplasm: Due to the tumor heterogeneity and the various analysis settings in different pipelines, we did not observe same gene alternation for all the genes detected, particularly for some low AF mutations (such as ALK G689R with an AF of 1.0% and of 1.2%, and KRAS Q61L with an AF of 0.1%) and rare mutations (like FGFR1 R455H, which is not reported in COSMIC).