To investigate the effects of detected mutations on the Hedgehog pathway gene expression, we quantified the expression of genes PTCH1, SMO, GLI3, and CCND1 in BCC (N = 20), NSS samples (N = 20) from NBCCS patients, and NS from healthy controls (N = 4). The gene discussed is GLI3; the disease is nevoid basal cell carcinoma syndrome.