The extent of gliosis was determined using GFAP staining in vehicle and drNPC transplanted mice at 32 days post-stroke (Fig. 6a) by measuring the maximal cortical GFAP+ area, which was strongly correlated (r = 0.89, n = 35, p < 0.001) to total gliosis volume per brain (Supplementary Fig. 4). The gene discussed is GFAP; the disease is stroke disorder.