Eight cases of RCC. The tissues were cut into 20-_m sections, stained with propidium iodide (PI) containing RNase A (final concentration, 0.5 mg/mL). There were a total of 50 slices for each volume data, and each slice was a 24-bit/pixel image with a size of 512 × 512 pixels. Here, RNASE1 is linked to renal cell carcinoma.