After transfecting miR-144 mimics and anti-miR-144 mimics into SW620 cells respectively, we further detected mRNA levels and found that miR-144 mimics reduced the transcription of SMAD4 (P<0.01, Figure 6B), conversely, anti-miR-144 mimics increased the transcription level (P<0.01, Figure 6B), which demonstrated that miR-144 might directly target SMAD4 in colon cancer cell lines. The gene discussed is SMAD4; the disease is malignant colon neoplasm.