Interestingly, if treated with recombinant protein HMGB1 (rHMGB1), the tumor cells released more ROS (from ~ 18.1% to ~ 36.5%), ROS released from tumor cells treated with rHMGB1 was also inhibited by GSH (reduced to ~ 14.4%) (Fig. 4i. The gene discussed is HMGB1; the disease is neoplasm.