To support that SMAD4 intercedes the part of miR‐130a‐3p in TGF‐β‐induced answers in ESCC cells, we transfected pcDNA3.1‐SMAD4 plasmid into EC‐1 cells with TGF‐β stimulation as well as miR‐130a‐3p overexpression. The gene discussed is SMAD4; the disease is esophageal squamous cell carcinoma.