Prior to this study, we have developed a clinically relevant syngeneic orthotopic mouse model of lung cancer which allowed the evaluation of tumorigenicity and metastasis of LLC‐SD with Nr5a2 depletion in C57BL/6 mice (Figure 4A).15 We employed this model to evaluate the relationship between the self‐renewal promoting effect of Nr5a2 that we observed in vitro (Figures 2E and 3C) and the cancer biological properties in vivo. Here, NR5A2 is linked to lung cancer.