In addition to the experimental R/K6.32 to alanine mutants, we have also performed mG BRET experiments using the naturally occurring cancer mutants FZD6 R416Q6.32 and SMO R455H6.32, as well as FZD6 W493L7.55 and SMO W539L7.55 (Supplementary Figures 6d, 8a, b). The gene discussed is SMO; the disease is cancer.