Next, we co-transfected CRC cells with expression plasmids of YY1, HDAC2, and p300, either alone or in combination with YY1 + p300, HDAC2 + p300, and YY1 + HDAC2 + p300, on the reporter gene plasmid pGL3-miR-500a-5p site 1. The gene discussed is YY1; the disease is colorectal carcinoma.