In fact, for several NLT markers we observed more cells expressing them in the tumor-draining LN compared to the control, e.g., Id2 (59% versus 26%), Batf (57% versus 26%), and Lgals1 (89% versus 67%), further supporting our hypothesis that there is priming of Treg cells to NLTs while still in the LN. This evidence concerns the gene LGALS1 and neoplasm.