We therefore purified Treg and Tmem cells from B16.F10 melanomas or PBS controls 11 days after subcutaneous implantation into Foxp3-IRES-eGFP reporter mice (Haribhai et al., 2007) to produce a plate-based scRNA-seq dataset (Figure 4A; see Experimental Procedures). The gene discussed is FOXP3; the disease is melanoma.