The EMSA, luciferase reporter assays and western-blotting demonstrated that NFκB activity in ESCC cells was significantly increased by exogenous FMR1-AS1 and suppressed upon FMR1-AS1 silencing (Fig. 4k-m), strongly suggested that FMR1-AS1 is involved in NFκB activation. This evidence concerns the gene NFKB1 and esophageal squamous cell carcinoma.