Therefore, by stably silencing TLR7 in ESCC cells, we found that the inhibition of TLR7 could block the activation of NFκB-c-Myc signaling pathway and suppressed the xenografts growth phenotypes by FMR1-AS1 exosome incubation (Fig. 6a-c, Additional file 11: Figure S6a and Additional file 4). This evidence concerns the gene FMR1 and esophageal squamous cell carcinoma.