We next examined both peripheral immune tissues (LN, spleen, BM) as well as tumor-infiltrating leukocytes (TILs) in untreated and anti-PD-1-treated hu-CB-BRGS mice by flow cytometry to evaluate human T, B and myeloid subsets, which have been shown to differ from the human chimerism in the blood and to change over time [20]. Here, PDCD1 is linked to neoplasm.