To evaluate the anti-tumor effects of LEF1-AS1 silencing in vivo, we established A549 cells stably expressing sh-control or sh-LEF1-AS1 We found that tumors with sh-LEF1-AS1 demonstrated significantly smaller sizes (Fig. 5a), as well as decreased Ki-67 expression comparing with the control (Fig. 5b).,Meanwhile, the tumors transfected with sh-LEF1-AS1 also exhibited an we obsversed obvious reduction of LEF1-AS1 expression and a prominent increase of miR-544a expression (Fig. 5c and d). The gene discussed is LEF1; the disease is neoplasm.