Thus our study stalwartly demonstrated that NVD has convincing anti-proliferation activity in human colon cancer cells, encouraging apoptosis and the anti-proliferation effect of NVD may be mediated by PI3 K/Akt signaling by blocking Wnt/β-catenin signaling transduction, through inhibiting the β-catenin expression respectively. The gene discussed is AKT1; the disease is colonic neoplasm.