In tissue specimens from xenografts, the decreased expression of β-Catenin, and down-regulation of Cyclin D1 and caspase 3 cleavage symbolize an encouraging validation of the NVD and FH535 potential in vivo in tumors inhibition and present adequate direct confirmation for the reticence of the canonical Wnt/β-Catenin pathway and of β-Catenin target genes by NVD and FH525 in human CRC. This evidence concerns the gene CCND1 and colorectal carcinoma.