CD8A and neoplasm: Flow cytometry analyses revealed the successful induction of B16 reactive Pmel-1 CD8+ T cells following aCD4 mAb treatment in the dLN CD44hi; in the aCD4 group, the frequency of Pmel-1 T cells tended to increase in dLN CD44hi (control; 1.9 ± 0.8%, aCD4; 4.5 ± 1.4%, P = 0.18), however, the frequency did not change in the tumor (control; 0.20 ± 0.12%, aCD4; 0.22 ± 0.10%, P = 0.91) (Figures 1E,F).