Using NGS on invasive pituitary adenomas and 6 non-invasive pituitary adenomas, 15 genes with pathogenic mutations were identified (131), including EGFL7, LRP1B, MGAM, MAST4, DSPP, PRDM2, PRDM8, ZNF717, LRRC50, TRIOBP, MX2, DPCR1, PRB3, SPANXN2, and KIAA0226. This evidence concerns the gene RUBCN and pituitary gland adenoma.