Moreover, we showed that CDK6 expression both in mRNA and protein levels were markedly restrained after transfecting with si-circ_0081143, and the inhibitory effects of si-circ_0081143 could be obviously reversed by co-transfection with miR-646 inhibitors (miR-646-in) in GC cells (Fig. 5b, c). This evidence concerns the gene CDK6 and gastric cancer.