Among the 23 subjects in the HL group with one p.V37I allele detected on the first screening step, four subjects had pathogenic or uncertain significance variants in other deafness genes unrelated to GJB2 (HL-63, SLC26A4:T410M/IVS7-2A > G; HL-49, MYO7A: p.G25R/ p.W1745R; HL-34, EYA1:p.S343C; HL-44, TMC1: p.L202V/p.T206S) (Table 1). Here, EYA1 is linked to deafness.