Among the 23 subjects in the HL group with one p.V37I allele detected on the first screening step, four subjects had pathogenic or uncertain significance variants in other deafness genes unrelated to GJB2 (HL-63, SLC26A4:T410M/IVS7-2A > G; HL-49, MYO7A: p.G25R/ p.W1745R; HL-34, EYA1:p.S343C; HL-44, TMC1: p.L202V/p.T206S) (Table 1). The gene discussed is GJB2; the disease is deafness.