According to the hot spots of p53 gene mutation verified, vector, wildtype and the most hot mutant spot 273 of p53 lentivirus were synthesized, and then transfected into the lung cancer H1299 cells (null of p53) to establish stable transfected cell clones respectively (p53 protein expression in these cells, Fig. 2d). The gene discussed is TP53; the disease is lung carcinoma.