Applying sample- and protein fragment-specific cut-off levels, a significant difference in the frequency of IgG-reactivity was identified for four antigens representing KRTAP17-1; heat shock protein family A (Hsp70) member 4, HSPA4; S100 calcium binding proteins A12, S100A12; and S100Z, differentiating between AD and HC or HC and any of the two subgroups of mAD and sAD (Table 2 and Additional file 1: Table S2). Here, HSPA4 is linked to Alzheimer disease.