After collecting together data from both the qPCR and RNA-Seq assays, we further evaluated in the total cohort of the study (48 T2D patients and 40 controls): (i) the levels of individual transcript variants of the genes found to be differentially expressed by qPCR (CDK5, CDKN2A, TSPAN8; Table 3 and Fig. 1A) and (ii) the levels of certain transcript variants found to be differentially expressed in RNA-Seq experiments (Fig. 1B and Supplemental Table 2). The gene discussed is CDK5; the disease is type 2 diabetes mellitus.