Larger number of hotspots and non-hotspots were also detected in GENIE in the NSCLC dataset for genes such as EGFR (738 versus 122, p < 0.0001), NF1 (211 versus 131, p < 0.0001) and PIK3CA (237 versus 94, p-0.038) in comparison to TCGA. Here, PIK3CA is linked to non-small cell lung carcinoma.